Description of the video:

00:05
hi everybody lindsay rasnake here with
00:07
the indiana clean lakes program to
00:09
introduce you to the first in a series
00:11
of videos we've created to help with
00:13
training on water quality monitoring
00:16
activities within our volunteer program
00:18
videos will serve as
00:20
an introduction to some of the water
00:22
quality monitoring activities we do or
00:24
can also serve as a refresher
00:26
that you can use from year to year as
00:28
you get back into the swing of water
00:29
quality monitoring so in this first
00:31
video we'll be going over how to take a
00:33
secchi disk measurement so i'll give a
00:35
quick overview and then we'll continue
00:38
on to an actual field demonstration of
00:39
how to use a secchi disk
00:41
a secchi disk is a
00:44
black and white disk much like the one
00:46
that i have with me here and it's
00:48
usually attached to
00:49
a measuring tape or a chain
00:52
or just something that is measurable as
00:54
you lower it into the water the secchi
00:56
disk gives us a measurement of water
00:58
clarity and is one of the simplest and
01:00
most straightforward tools that we have
01:02
for water quality monitoring
01:04
so our volunteer securities are attached
01:06
to measuring tapes which are in feet and
01:08
tenths of feet and this just makes it
01:10
easier
01:11
when recording it reduces the amount of
01:13
error and it's just easier to read as
01:15
you're lowering the disc into the water
01:17
so to collect a sucky measurement you're
01:19
going to lower the disc slowly into the
01:21
water until the black and white
01:25
strips are no longer visible on your
01:27
disk and then you're going to record
01:29
that measurement
01:31
and then you're going to start to raise
01:33
it back up just until you can see the
01:35
disc again and record that measurement
01:37
as well the average of these two depths
01:39
is going to be your actual secchi
01:41
measurement
01:42
so the secchi measurement tells us about
01:44
water clarity the greater the depth of
01:46
the secchi the clearer the water
01:50
so when collecting a secchi measurement
01:52
the user is also going to record some
01:54
qualitative observations such as the
01:56
date that they're taking the measurement
01:58
the time as well as the general um
02:02
aesthetic of the water the recreation
02:04
potential of the lake and things like
02:05
that this gives us some additional
02:07
context into the actual quantitative
02:10
measurement of the secchi
02:12
after collecting secchi data the
02:14
volunteers will then use our online data
02:16
entry form to send in all the data that
02:19
they've collected for that particular
02:20
sampling event or they can wait until
02:22
the end of the season and enter all of
02:24
their data at once
02:26
then at the end of the year we'll
02:27
compile all of that data and release it
02:29
back to the volunteers
02:31
more information about what the secchi
02:33
measurement tells us is available on our
02:34
website under the lake information and
02:37
data tab so be sure to go to that and
02:39
view our document about interpreting
02:40
lake data just to get
02:42
some more background information and a
02:44
general overview of what secchi
02:45
measurements can tell us
02:48
continuing in this video we have like i
02:49
said a field demonstration of using a
02:51
secchi disk so continue on to watch that
02:54
and happy sampling so um like i said
02:57
after you drop your anchor the first
02:59
thing you want to do is go ahead and
03:00
collect your secchi measurement
03:02
so for this you want to make sure that
03:05
you have
03:06
your disc as well as your data sheet
03:08
ready to go
03:11
that's just to keep those separated
03:13
um so before you take your
03:16
seki the other thing you want to have
03:17
ready to go is your little cheat card
03:19
which will tell you your starting volume
03:22
that you're going to filter for
03:23
chlorophyll
03:25
so
03:27
before starting you want to make sure
03:28
that you filled out just some general
03:30
information on your data sheet your lake
03:33
name the date that you're sampling the
03:35
time you're sampling right now we're
03:37
close to 2 15. it's okay to round up a
03:39
couple of them and then um after you've
03:42
written your sample time we're ready to
03:43
get going
03:47
so
03:48
when collecting a secchi
03:50
it's good practice to do it on the shady
03:53
side of the boat sometimes like at
03:55
midday that's kind of hard to do and
03:57
that's all right um but like for this
04:00
instance this side would probably be
04:02
better than that side i would say
04:05
um and then also if you're like me and
04:07
you're wearing a hat or sunglasses you
04:09
want to take those off for while you're
04:10
collecting the seki i just like to turn
04:12
mine around um because that will affect
04:16
um the vision of your of your secchi
04:18
disc
04:19
so it's okay
04:21
so um i don't ever everyone can come
04:23
over and look um
04:26
if
04:27
we don't tip me over um so you're going
04:29
to lower it slowly into the water um you
04:32
don't want to go too fast because you
04:33
want it to be sinking um slowly
04:36
you still have to look directly over it
04:38
yep you're gonna so you're gonna look
04:40
directly just like i'm doing um
04:42
straight down at your disk
04:44
and then um you're going to
04:47
take a measurement where it disappears
04:50
this is going to be different for
04:50
everyone so right here it's gone for me
04:54
oh actually yeah i can do a little bit
04:56
my shadow is kind of over it so that's
04:57
the other thing is if your shadow is
04:59
over it that's going to affect it as
05:00
well
05:01
so yeah
05:02
so this is probably where we would want
05:04
to take a measurement that's going to be
05:05
at the surface of the water so right
05:08
here we're at two point
05:11
about 2.3
05:13
um and it's okay if you're
05:16
you know um
05:17
0.01 off that's all right that's not
05:20
gonna cause a problem right now i can
05:22
see that there's just a little bit of
05:23
light down there but you can't really
05:25
make it out yeah so so it's it has
05:27
disappeared when you can't see
05:30
the like separation between the white
05:32
and the black anymore so when you can't
05:34
see that disc anymore
05:35
that's when you're going to take a
05:36
measurement and then you're going to
05:37
start to pull it back up
05:39
oh otherwise sorry you're going to start
05:41
to pull it back up and then take a
05:42
measurement when you can see it again
05:44
so at this point
05:45
i can see it i can see it again so i'm
05:47
going to take a measurement and i'm at
05:49
2.2
05:50
so that is gonna be really really close
05:53
it shouldn't be too far off point one
05:55
off is about what you're gonna be should
05:57
we have reach the two measurements so
05:58
we're gonna average exactly we're gonna
05:59
average those two and then write that on
06:02
our data sheet
06:04
yep exactly

Description of the video:

00:05
hello again lindsey rasnake here with the
00:06
indiana clean lakes program to introduce
00:08
the second in our series of training
00:10
videos for new volunteers or current
00:13
volunteers that might need a refresher
00:15
on some of the sample collection
00:16
processes
00:18
if you haven't already watched our
00:19
secchi disc video be sure to watch that
00:21
one first so that you can get a little
00:22
bit of an overview or refresher on
00:24
collecting secchi measurements
00:26
in today's video we'll be talking about
00:28
how to use an integrated sampler to
00:30
collect water samples for analysis in
00:32
our lab
00:34
our expanded monitors which collect
00:36
these water samples measure for total
00:37
nitrogen total phosphorus and
00:39
chlorophyll a once a month for
00:42
four months out of the year may to
00:44
august
00:44
our volunteers collect these water
00:46
samples in addition to their bi-weekly
00:48
secchi measurements
00:50
so be sure to go over our interpreting
00:52
lake data document on the lake data
00:54
information tab on our website to
00:56
familiarize yourself with some of these
00:58
water quality parameters and what they
00:59
tell us about water quality across our
01:02
state
01:03
so the integrated sampler which i have
01:05
with me here is a two meter length of
01:07
pvc pipe with a ball valve at the end
01:10
the sampler is constructed to integrate
01:12
the full first two meters of the water
01:15
column so we're getting a fully mixed
01:19
sample of the surface water
01:22
so users of the integrated sampler will
01:23
begin by opening their ball valve about
01:25
90 of the way open or at least as open
01:28
as they can get it while still being
01:30
able to actually pull the valve closed
01:32
they won't be able to open it all the
01:34
way as they won't be able to pull the
01:36
rope to have it shut so it needs to be
01:38
at just a little bit of an angle so that
01:40
you're able to shut it
01:42
so after rinsing the integrated sampler
01:44
on the opposite side of the boat from
01:46
where you'll actually collect your
01:47
sample you're ready to collect
01:50
you'll lower it slowly down into the
01:52
water until it reaches the 2 meter mark
01:54
on your integrated sampler
01:57
from there
01:58
you'll hold
01:59
the stopper with one hand while you pull
02:01
the rope to shut the ball valve with the
02:03
other make sure that as you're lowering
02:05
you're going as slow as possible so that
02:07
you're able to integrate the full two
02:09
meters of the water column
02:11
once you pull the sample up you're ready
02:14
to release the ball valve so that you
02:15
can pour the water into your pitcher
02:18
this is what you'll be using to fill the
02:20
rest of your samples okay so continue
02:22
watching for a field demonstration of
02:24
how to use the integrated sampler and
02:26
happy sampling
02:40
okay
02:41
so that is all quick rinse and then
02:43
we'll rinse our integrated sampler
02:46
um again you want to kind of make sure
02:47
this is turned at an angle
02:50
and you can sort of
02:52
feel how open it is you want it to be as
02:54
open as you can get it
02:56
um and still be able to pull it shut you
02:58
won't be able to do it like from right
03:00
here
03:01
because there's just no way but just a
03:03
slight
03:04
angle so um i don't know if all of you
03:06
can see this
03:08
but what i like to do is when i lower it
03:10
down i put my hand on top of it my left
03:12
hand on top and my right hand on the
03:14
handle
03:15
and then i just pull up to shut it
03:22
and then
03:23
just open to release
03:24
rinse it out
03:27
once you've rinsed it i wouldn't put
03:29
your fingers in there to check because
03:31
then you contaminated it we carry a lot
03:33
of phosphorus
03:35
so um and since we're worried about
03:36
phosphorus with this testing we want to
03:38
minimize that as much as possible
03:40
so then we'll collect our staple over
03:42
here sorry yeah and then so what i was
03:44
saying was when i put it down i like to
03:46
keep my left hand on top and then i use
03:48
the i pull the rope with the front
03:51
but whatever works best for you and
03:53
again i'm not trying to get fresh
03:55
you want this two meter mark to be at
03:57
the water it's okay if it's like right
03:59
here you just want it to be as close to
04:01
two meters as possible
04:09
don't fall in
04:11
i almost did the other day
04:12
the other thing is when you lower it in
04:14
you want to go pretty slow
04:16
someone have my picture oh yeah right
04:18
here
04:19
you want to go pretty slow because like
04:21
i mentioned it's kind of like a straw
04:23
um and so you want to wait for that
04:25
whole column to be drawn up into the
04:28
sampler
04:31
and you can see that one sample pretty
04:33
much filled my picture yeah so we
04:35
probably won't need to collect another
04:36
one but even if we do
04:39
we'll have to wait until we've empty
04:40
this to collect another pretty green
04:43
yeah

Description of the video:

00:04
hello again lindsey here with the
00:06
indiana clean lakes program in the third
00:08
part of our series of training videos be
00:10
sure to refer back to the first two
00:12
videos to see our demonstration of using
00:15
a secchi disk as well as an integrated
00:17
sampler
00:18
in today's video we're going to be going
00:19
over the actual process of pouring
00:22
samples into our sample bottles and the
00:24
filtration process for chlorophyll
00:26
volunteers will receive two bottles each
00:28
month a 125 ml bottle for total nitrogen
00:32
and total phosphorus as well as an
00:34
opaque 60 ml bottle for your chlorophyll
00:36
filter
00:39
so once you've collected your integrated
00:41
sample and poured it into your pitcher
00:44
you're going to be ready to fill your
00:46
sample bottles for the total nitrogen
00:48
total phosphorous bottle you don't have
00:49
to do much you just want to give your
00:51
sample a good mix and then pour it into
00:53
the bottle just up to the shoulder
00:57
after that you're ready to do
00:59
your chlorophyll filtration process for
01:02
which you're going to be using a
01:04
filtering apparatus
01:06
and a handheld pump
01:09
so the goal is to filter enough water
01:10
through so that you have chlorophyll
01:13
accumulating on your actual filter paper
01:17
these are sent to the volunteers each
01:18
summer with enough
01:20
in their packets to sample all season
01:29
you'll be using your own set of tweezers
01:31
to take your filter paper and put it on
01:34
your filtering apparatus
01:42
once you've got your filter loaded onto
01:43
your filtering apparatus you're ready to
01:45
actually start filtering so
01:47
since you've already collected your seki
01:48
measurement you can use your nifty cheat
01:52
sheet card which will tell you according
01:54
to the secchi depth about how much volume
01:56
of water you're going to be filtering
01:58
this is just a starting point so you
01:59
don't have to stay to this you can
02:01
always filter more water but you just
02:03
want to be able to get enough
02:04
chlorophyll on the filter that we can
02:06
actually measure it here in the lab on
02:08
the other side of the card we also have
02:09
a guide for how to see how much
02:12
chlorophyll you've got on your filter
02:13
and if it's enough so you always want to
02:15
have it at this really nice dark green
02:17
so that there's plenty of chlorophyll
02:18
for us to measure
02:21
once you know how much volume you're
02:22
going to filter
02:24
you're going to use your 250 ml
02:26
graduated cylinder to measure out that
02:28
water we need a very precise volume so
02:30
always make sure you record exactly the
02:32
volume that you filter
02:34
when filtering always make sure that
02:37
all of the water has been filtered
02:38
completely through the apparatus before
02:40
you actually extract your filter paper
02:43
additionally
02:45
when pumping you don't want to go above
02:47
seven psi of pressure on your hand pump
02:50
if you go above this you run the risk of
02:52
breaking your filter
02:54
when you're ready to extract your filter
02:56
paper you can use the lever on your hand
02:58
pump to release all the pressure back to
03:00
zero at that point you can remove the
03:02
hand pump from your filtering apparatus
03:04
you can unscrew the top part of the
03:07
apparatus
03:09
and while trying to minimize as much
03:11
contact with sunlight as possible you
03:13
can extract your filter paper
03:15
and when doing this you want to fold the
03:17
paper over
03:20
in half
03:21
three times
03:25
before putting it
03:27
into your opaque bottle
03:30
after collecting these you always want
03:31
to make sure that you've written your
03:33
sample dates as well as the volume you
03:34
filtered for chlorophyll on your sample
03:36
bottles
03:37
immediately after collecting these you
03:39
want to put them in the freezer until
03:40
they're ready to be shipped to our lab
03:42
so with this overview of the process
03:44
you're ready to watch our field
03:45
demonstration of how to collect a tntp
03:48
sample and filter for chlorophyll i want
03:50
to thank you all for staying tuned on
03:51
this series of training videos and let
03:53
you know that you can always reach out
03:54
to us by phone or email with any
03:56
questions or concerns that you have if
03:58
you're interested in being a volunteer
04:00
on your lake please reach out to us and
04:02
we'll get you set up to start collecting
04:03
data
04:05
lastly we want to thank you all for
04:06
always working hard to keep indiana
04:08
lakes clean yet today let's talk a
04:10
little bit about this we just want to
04:11
make sure that we've kind of got in mind
04:13
the volume that we're gonna filter for
04:14
chlorophyll um and at this point you can
04:17
i'm gonna put my hat back on
04:23
so at what was it 2.25 2.25 so we're
04:26
going to be in the um 1.5 to 2.5 foot
04:30
range
04:31
so that's going to be starting at about
04:33
200 mils of water to filter
04:35
first we're going to rinse
04:37
we're going to rinse all of our
04:38
equipment
04:40
um and i actually recommend
04:42
because it's easier if we sample over
04:44
here let's rinse our stuff over there a
04:47
few things to rinse
04:53
so we're going to start with
04:54
all of our equipment that we're going to
04:56
put our sample water in um for the
04:58
filtering apparatus
05:00
we don't do anything with the water once
05:02
it's been filtered um so i'm not worried
05:04
about rinsing this
05:06
because it doesn't matter
05:07
but we do want to rinse the top
05:11
there are some pieces that are kind of
05:13
small and so you just want to be careful
05:15
when rinsing i would just rinse one
05:16
thing at a time oh thank you
05:19
i appreciate it
05:21
um and it's good practice to do three
05:23
rinses
05:24
of everything
05:28
so a question
05:29
so you don't drop stuff over the boat
05:32
grab a five gallon pail and just bring
05:34
up enough water and then
05:36
you you could do that as well um i would
05:39
rinse your pail first
05:41
and then yeah you could do that but
05:42
usually this piece has a little um
05:44
o-ring so and as you can see they can
05:47
fall out pretty easily so you just want
05:49
to be mindful of that
05:50
when you're rinsing and i like to make
05:52
sure my lid is screwed on tight and then
05:54
i just rinse it i just fill it and dump
05:56
it
05:59
and most of this floats but not all of
06:01
it
06:02
thank you
06:15
okay
06:17
so now i'm ready to put my filter on
06:19
once i rinsed it and got the filter
06:21
plate a little wet the filter will seat
06:23
better
06:24
things to keep in mind with this filter
06:26
plate is that this piece comes out
06:29
and sometimes people forget if it goes
06:30
this way or goes this way
06:32
it does need to go with the rough side
06:34
up so you can see this one's smooth
06:37
and this one's rough
06:39
so you want it to be rough side up or
06:41
else it won't pull through very well so
06:43
you'll notice really quickly if you got
06:45
it flipped the wrong way it just you
06:46
won't be able to filter water through
06:48
and if that happens you'll have to dump
06:50
it
06:51
and start over does it have a lip on it
06:54
like
06:55
the filter itself or is it still around
06:57
i mean can you put it in the other way
06:59
that's just this way
07:01
it'll fit in but it doesn't it doesn't
07:03
see it doesn't seat very well so yeah
07:05
you'll know from that way too if this
07:06
fits flush with the the rest of those
07:09
yeah
07:11
so we want to set it here and now we're
07:12
ready
07:13
um to put our filter on when opening
07:16
these um these filters are really light
07:18
so it's really easy for the wind to blow
07:20
them away what i like to tell people is
07:22
open it pac-man style
07:24
just kind of like this
07:27
and then just pull use your forceps to
07:29
pull one filter out
07:31
then go ahead and shut it put it away
07:33
and then you're just going to set your
07:35
filter on top of your filter plate like
07:37
i said with it being wet which i touched
07:39
it a little bit so it dried it out some
07:42
but with it being wet it'll help it seat
07:44
you just want to put it in the middle um
07:46
so that you can catch all of your
07:49
chlorophyll and you don't get any water
07:51
coming through there
07:52
so then i'll take this lid off really
07:54
quick
07:55
when you're ready to screw down the top
07:58
part of your filtering apparatus you
07:59
notice
08:00
it's loose
08:02
you want to screw this white part down
08:04
but if you've screwed it down and it's
08:06
still jiggling like this it's not seated
08:08
properly
08:09
so you want to make sure that once
08:11
you've screwed it down it's threaded so
08:13
that this top piece is not jiggling okay
08:16
do you see you guys see the difference
08:17
between
08:18
yeah so it should be firm it shouldn't
08:20
jiggle if um it does you will lose
08:22
sample so you just want to make sure
08:24
that that's screwed on tight
08:26
okay
08:29
a graduated cylinder so um like i said
08:31
this graduated cylinder is marked at 250
08:34
mils it's a little bit hard to see
08:37
um but you also like once you've done it
08:39
several times you kind of get a sense
08:40
for where the 250 mark is
08:42
so our starting point is 200 so i'm
08:45
gonna go ahead and measure to 200 and
08:47
you'll notice that um
08:50
the uh the middle lines like it goes 190
08:52
210 230 so just keep in mind that those
08:55
middle lines are going to be the 200 the
08:57
100 etc um so just keep an eye out for
09:00
that you could mark your fridge with a
09:02
level with your sharpie yeah sure if it
09:05
was tough for you to see
09:06
feel free to mark it with sharpie
09:09
before you pour any sample you want to
09:11
make sure to always mix it
09:14
[Music]
09:15
and that just
09:16
keeps anything that's suspended in it
09:18
from going straight to the bottom you
09:20
want to keep it all suspended as evenly
09:22
as possible i like to tilt
09:24
my cylinder when i'm pouring i just
09:26
think it's a little bit easier
09:28
you do it however works for you
09:30
if you over fill it a little bit
09:34
that's okay
09:35
just write down whatever volume you
09:36
ended up filtering
09:38
if you over filter it a lot like i just
09:40
did to 210 that's okay i'm just going to
09:42
go ahead and filter that whole 210. once
09:45
i've poured in here
09:46
i have to filter everything i poured in
09:48
here or i need to dump it and pour more
09:50
so you can't just pour a little water
09:52
okay don't pour any off um because
09:55
you're you could be losing sample um and
09:57
you don't want to do that so you want to
09:59
you want to be as precise as possible
10:00
i'm doing this okay
10:02
um also thing to keep in mind is since
10:05
chlorophyll is light sensitive you want
10:07
to kind of keep it as shaded as you can
10:10
since it's pretty overcast right now
10:11
we're not overly concerned but like if
10:14
it was really sunny i might even turn my
10:16
body so that my body is shading yeah or
10:19
you can enter the shade yes
10:21
you can always do all of this in the
10:22
boat right when you've done it you don't
10:24
take the picture back to you i wouldn't
10:26
i just go ahead and do it that way if
10:27
you need to get more you can get more
10:28
that's good yeah so i'm gonna pour it in
10:35
and i like to just go ahead and set this
10:37
on top of it you can screw it in if you
10:39
want
10:42
then i'll hook my hand pump up
10:48
and as i'm pumping you can see oh yeah
10:51
it's pulling
10:52
start to pour water down through there
10:54
and that's about the stream it'll go in
10:56
if there's a lot of um
10:58
chlorophyll in your sample it will go
11:01
slower the more you collect on that
11:03
filter the slower it's going to go so
11:05
towards the end it's yeah it's going to
11:06
go slower because you're collecting on
11:08
that filter
11:09
um and again i want to make sure i'm not
11:11
going above seven psi right now i'm i'm
11:14
at about six
11:15
everyone can see that like i said it's
11:17
the black numbers on the outside
11:19
um and once i've built up pressure i can
11:20
kind of let it sit for a little bit
11:23
so i'm gonna go ahead and take this off
11:25
just to look at my filter and see how
11:27
green it is remember when we go to pull
11:29
the hand pump off we want to make sure
11:30
all the pressure is out of it so i hit
11:32
this little lever and now the pressure
11:34
is at zero okay and i'll move this so
11:36
you guys can see better sorry about that
11:38
um again like i want to kind of keep it
11:41
shaded
11:42
yeah as much as i can
11:46
so this is and this is just a practice
11:47
this is not the sample we're going to
11:49
take but um so you can see that's still
11:51
like decently light there's some brown
11:53
on there it will be a little green brown
11:55
it won't necessarily be bright green
11:57
every time
11:58
but i might go ahead and filter i don't
11:59
know maybe 50 more
12:02
and also keep in mind that you're
12:04
you're well
12:06
i think you guys have a bigger one so
12:07
you have a leader here
12:09
i think i only have 500. okay so um if
12:13
you need to filter more than a liter you
12:14
fill the whole thing you're obviously
12:16
going to need to dump it before you can
12:18
filter any more
12:20
and so if you do that that's okay you
12:22
can just
12:23
take this top piece off make sure that
12:26
you kind of
12:27
keep your filter plate covered
12:30
and just sort of set it like this dump
12:32
your water doesn't hurt
12:34
no touching the bottom
12:36
won't do anything to it touching the
12:38
sides is fine because i just don't i
12:40
want to kind of keep my fingers off of
12:42
the top of the filter as much as i can
12:44
but i can touch the sides
12:46
set it down all that just try to keep it
12:48
out of the sun
12:49
so so if you set it over it like this
12:52
that's totally fine it's protected from
12:54
the sun at that point
12:55
um
12:58
so then i'm just gonna go ahead and
13:00
filter
13:02
50 more so that you guys can see that
13:04
process so we want to do a mix
13:07
yep so we are just making sure
13:09
everything stays suspended
13:11
we want to do about 50. oh i went high
13:14
that's okay what'd you get 60. that's
13:16
okay
13:17
so
13:18
if i'm pouring 60 into here i did 210
13:20
the first time i'm gonna be at 270. you
13:22
want to keep that in our heads or if you
13:24
want to keep a little scrap piece of
13:25
paper that's fine too
13:27
just keep in mind of how much you're
13:28
filtering
13:30
so if you didn't have the vacuum pump
13:31
would it still
13:33
filtered really slowly yeah and probably
13:36
not all of the water would go through
13:38
oh look it is going yeah
13:40
you've already started to come back
13:45
i'm about seven i can set it down and
13:48
while i'm waiting for it to finish
13:49
filtering i can go ahead and fill my
13:51
tntp bottle if i want to
13:53
um same procedure for this
13:56
do a little bit of a mix make sure
13:57
you're not putting your fingers around
13:59
the rim of the bottle or anything like
14:00
that because like i said you have
14:02
phosphorus on your hands nine times out
14:04
of ten so you want to make sure to keep
14:06
that out of your sample
14:07
these are sterile so we don't have to
14:09
rinse them no don't have to do a rinse
14:10
they're acid washed before i send them
14:12
to you um and so they stay shut until
14:15
they get to you so they're okay and
14:16
there's no chemicals in them nope
14:18
they're just collection bottles nope
14:20
just collection bottles nothing in here
14:22
will hurt you okay if you do like maybe
14:24
you drop it and you accidentally touch
14:26
the lid that's okay you can go ahead and
14:27
do a sample rinse there's nothing wrong
14:29
with doing a sample rinse
14:31
i'm going to mix oh that's a good point
14:33
so there aren't any chemicals that we're
14:34
really interacting with
14:37
in any of these steps where we need to
14:38
wear protective
14:40
you're going to fill it to the edge
14:42
there what i call the shoulder kind of
14:44
see where it comes
14:45
over the reason why you're only feeling
14:47
to the shoulder not to the top is
14:49
because we freeze these
14:51
um and so we need a little bit of space
14:53
for the water to expand if you over fill
14:56
them they'll bust when we put them in
14:58
the freezer you all know this
15:01
um
15:02
just so that i've said it though
15:05
they will bust
15:07
um so then that's your sample for that
15:10
and now you um are done with your
15:12
chlorophyll
15:14
um again i want to make sure that i'm
15:16
lining pressure off and you can hear it
15:18
just double check that it's come out
15:21
um
15:23
for me this is pretty this is pretty
15:24
good like i said this like it's a little
15:26
bit brown and that's okay you just want
15:28
to make sure that you have enough
15:29
material on there so that you can filter
15:32
by all means you can filter more than
15:34
this if you want to if you wanted to go
15:36
ahead and do 300 that's okay too you
15:38
just need to know how much was it
15:41
how much exactly exactly
15:43
so once i filtered enough again this is
15:45
practice honestly if i was out here
15:47
doing this i might go ahead and filter
15:49
30 more just to make it to an even 300
15:51
because it won't hurt it to do more and
15:53
it was still going through pretty quick
15:55
so you could do more
15:56
oh
15:58
so okay so i folded it over once and
16:00
then once i folded it over i can put my
16:02
finger on it because i'm touching the
16:03
back of the filter there's no material
16:05
on the back of this filter
16:07
so i folded it over once i'm going to
16:09
fold it over again
16:12
and then i'm going to fold it over one
16:13
more time someone i trained a couple
16:15
weeks ago called this a pizza fold yeah
16:18
so sure pizza fold
16:20
and then you're gonna take your opaque
16:22
bottle mine's blank yours is gonna be
16:24
labeled of course um so you're gonna
16:27
take that
16:28
don't have to worry about touching the
16:30
rim there's nothing on your hands that's
16:31
gonna hurt this
16:32
um
16:33
and then you're gonna go and put your
16:34
filter in try and keep it away from the
16:37
sunlight as you're pulling it out as
16:38
much as possible
16:40
um do you freeze it too yes so that's
16:43
another thing i was gonna say once
16:44
you've collected your samples um if
16:46
you're gonna be out on the water for a
16:47
while i would take a cooler out with
16:50
some ice in it so you can put these on
16:51
ice
16:52
it's
16:53
for both of them it's pretty important
16:55
to keep them as frozen as possible
16:58
for as long as possible before they get
16:59
to us
17:00
if you're going straight back to your
17:02
house probably not as much of a concern
17:03
but as soon as you get back go ahead and
17:05
stick them in the freezer
17:06
and then if it's your first month like
17:10
for example i'll send out may june
17:12
bottles at the end of april so if once
17:15
you've collected in may
17:16
you would go ahead and freeze those then
17:18
you'll collect in june
17:20
freeze those and then you'll send them
17:21
back to me and you'll label them
17:24
yes
17:25
they will be labeled yes um so speaking
17:27
of on the label you're gonna fill in the
17:29
date that you sampled and then you're
17:31
going to fill in for chlorophyll the
17:32
volume that you filtered you're also
17:34
going to write the volume you filtered
17:36
on your data sheet so for example we'd
17:38
write 270 on there and then we'd write
17:40
270 on here
17:43
good
17:48
you